There are little things that are functional and help cities gain a reputation. For example, New Orleans and food & culture or San Francisco and the Trolley. To some it may not make much difference but you will often find these symbols plastered all over billboards, advertisements and media. Eventually, they become tied to the identity of the place.
So trolleys can help put flavor on an existing brands but may not be the only differentiation. The totality of images, impressions, and cultural feel will create a theme of which trolleys fit like to create a "scene". So when I think of New Orleans there are a number of images all connected together through my five senses and the way information is coded.